STUDIES ON PITUITARY LACTOGENIC HORMONE
نویسندگان
چکیده
منابع مشابه
Studies on Pituitary Lactogenic Hormone
The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility of the hormone at different hydrogen ion concentrations in acetate and phosphate buffer of ionic...
متن کاملStudies on Pituitary Lactogenic Hormone
Previous investigations have shown that the ovine prolactin is composed of a single chain at the N terminus, with threonine as the terminal residue (1). Since the hormone protein has 3 cystine residues (2, 3), it might be anticipated that one or more of these three -S-Sbridges may form a branched chain in the C terminus or along a portion of the polypeptide chain. If this were the case, oxidati...
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Northrop and coworkers (1-3) believe that solubility studies constitute the most sensitive and reliable test for determining the homogeneity of proteins and have used them successfully as a test for purity of their crystalline enzyme preparations. Herriott, Desreux, and Northrop (4) were able to show that a number of pepsin solutions that appeared to be homogeneous in electrophoresis experiment...
متن کاملSTUDIES ON PITUITARY LACTt)GEI’tIC HORMONE
In recent publications experiments were described demonstrating the homogeneity of our lactogenic hormone preparations by means of both electrophoretic (1, 2) and solubility (3) criteria. Of pituitary hormones the lactogenic seems to have been the first isolated in chemically pure form. Its molecular weight, however, has not yet been reported, although for many years it has been thought to be c...
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In various methods that have been reported for the preparation of pituitary gonadotropin, such as those of Jensen, Simpson, Folksdorf, and Evans (1) and Chow, van Dyke, and Greep (2), the active material is obtained either by precipitation with organic liquids or by salt precipitation, after which the gonadotropin or gonadotropins are separated from the precipitate. However, Fevold, Lee, Hisaw,...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65039-2